ABSTRACT
This study was aimed to establish the ultra high performance liquid chromatography (UHPLC) fingerprints of Psoralea corylifolia L. The separation was achieved on a Shim-pack XR-ODS Ⅲ column (50 mm í 2.0 mm, 1.6μm) by gradient elution with acetonitrile-0.2% glacial acetic acid solution as the mobile phase. The flow rate was 0.1 mL·min-1 and the measurement wavelength was 246 nm. The temperature of the column was 45oC. The results showed that the UHPLC fingerprint of P. corylifolia L. was established and 10 characteristic common peaks were found, among which 6 peaks were recognized by comparing with reference substances. It was concluded that the method was rapid, reliable and reproducible. The established fingerprint can provide references for the study of sub-stance basis and quality control of P. c orylifolia L.
ABSTRACT
Objective To investigate the measures to improve the newborn hearing screening quality.Methods835 cases of neonates using distortion product otoacoustic emissions for hearing screening,after first,second and third hearing screening,the final 5 cases failed,diagnosed by ABR screening hearing handicap in 3 cases.Experience constantly was summed up during screening process to improve screening quality.Results835 newborns were screened.The rate of failing to hearing screening was 0.60％,the rate of hearing impairment diagnosed by ABR was 0.36％,and the positive proportion of hearing screening was 0.24％.ConclusionOur experience during screening progress is to take some measures to improve the newborn hearing screening quality,with reducing false positives and avoiding false negatives,by choosing screening time,cleaning external ear and electric-otoscopy examination,environmental noise control,updating test procedures according to the physiology and development of the newborns,testing calibration instruments of otoacoustic emissions hearing screening,selecting suitable earplug,probe with correct plsce,and combining acoustic impedance examination,with a long term follow-up of listening in high risk neonates with passing screening.
ABSTRACT
Objective To observe the safety and effectiveness of high-dose chemotherapy(HDT) supported by autologous peripheral blood hematopoietic stem cells transplantation(APBHSCT) in the patients of terminal or relapsed malignant tumors.Methods The patients achieved CR or PR after 4 cycles of conventional salvage chemotherapy were divided into two groups randomly.26 patients in the research group were adapted APBHSC+HDC,50 patients in the control group were adapted 2～4 cycles of routine chemotherapy.Results In the research group,hematopoiesis was reconstructed in all patients,8 out of 17 patients who achieved PR after 4 cycles of conventional salvage chemotherapy turned into CR after APBHSC+HDC(CR rate 47.1%).In the control group,33 patients who achieved PR after 4 cycles of conventional salvage chemotherapy were still PR after 2～4 cycles of routine chemotherapy.The median survival period in the research group was 11 months,which was longer than the one in the control group.The survival rates of 2,3 and 4 years in the research group were evidently higher than the ones in the control group(P